{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE329nnn/GSE329306/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE329306"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Single-cell time-course transcriptomic analysis reveals epithelial remodeling in human gastric corpus organoids after WNT pathway perturbation","description":"WNT signaling is essential for gastric epithelial stem cell homeostasis. However, the temporal transcriptional dynamics following WNT/RSPO perturbation in human gastric organoids remain unclear. Human gastric corpus organoids derived from normal tissue were stabilized and then subjected to WNT/RSPO perturbation. Single-cell RNA sequencing was performed at 0, 6, 12, 24, 48, and 72 hours. Differential gene expression, pseudotime analysis, and pseudobulk profiling were conducted to characterize temporal changes. WNT/RSPO perturbation induced progressive epithelial remodeling, characterized by a decline in stem cell populations and expansion of tuft and mucous cell populations. This temporal single-cell transcriptomic analysis delineates distinct phases of transcriptional remodeling following WNT/RSPO perturbation and distinguishes direct WNT signaling responses from subsequent differentiation-associated changes in human gastric corpus organoids.","dates":{"publication":"2026/09/29"},"accession":"GSE329306","cross_references":{"GSM":["GSM9701556","GSM9701557","GSM9701555","GSM9701558","GSM9701559","GSM9701560"],"GPL":["24676"],"GSE":["329306"],"taxon":["Homo sapiens"]}}