GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE329nnn/GSE329306/primaryOK200TranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE329306GEOGSEfalseSingle-cell time-course transcriptomic analysis reveals epithelial remodeling in human gastric corpus organoids after WNT pathway perturbationWNT signaling is essential for gastric epithelial stem cell homeostasis. However, the temporal transcriptional dynamics following WNT/RSPO perturbation in human gastric organoids remain unclear. Human gastric corpus organoids derived from normal tissue were stabilized and then subjected to WNT/RSPO perturbation. Single-cell RNA sequencing was performed at 0, 6, 12, 24, 48, and 72 hours. Differential gene expression, pseudotime analysis, and pseudobulk profiling were conducted to characterize temporal changes. WNT/RSPO perturbation induced progressive epithelial remodeling, characterized by a decline in stem cell populations and expansion of tuft and mucous cell populations. This temporal single-cell transcriptomic analysis delineates distinct phases of transcriptional remodeling following WNT/RSPO perturbation and distinguishes direct WNT signaling responses from subsequent differentiation-associated changes in human gastric corpus organoids.2026/09/29GSE329306GSM9701556GSM9701557GSM9701555GSM9701558GSM9701559GSM970156024676329306Homo sapiens