{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE329nnn/GSE329314/"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE329314"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Radiation Induces Persistent Dysregulation and Functional Impairment in CD8+ T Cell Immunity","description":"In addition to immediate cell lethality, radiation exposure has longer term effects on the immune system, particularly T lymphocytes, causing persistent functional abnormalities post-exposure. Uncertainties remain regarding the long-lasting consequences on T cell immunity in acute-radiation-syndrome survivors and the underlying mechanisms. Here, we investigated delayed effects of acute radiation exposure on CD8+ T cell immunity using the Listeria monocytogenes infection model. Impaired CD8+ T cells activation, reduced terminal effector formation upon infection, and exacerbated listeriosis were found in mice at four weeks post-irradiation with sublethal doses. To elucidate how radiation affects the functionality of CD8+ T cells at various differentiation stages, we performed single-cell transcriptomic profiling. Irradiation altered cluster distribution pre-infection and impaired terminal effector and memory precursor effector formation post-infection. Differential gene analysis highlights radiation-induced gene expression changes, including cytokines and mitochondrial proteins, in a cluster-specific manner post-infection. The study demonstrates dysfunction of newly replenished naïve T cells, which results in impaired CD8+ T cell immunity after irradiation.","dates":{"publication":"2026/06/30"},"accession":"GSE329314","cross_references":{"GSM":["GSM9701630","GSM9701633","GSM9701634","GSM9701631","GSM9701632","GSM9701637","GSM9701635","GSM9701636"],"GPL":["34290"],"GSE":["329314"],"taxon":["Mus musculus"]}}