GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE329nnn/GSE329314/primary200OKTranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE329314GEOGSEfalseRadiation Induces Persistent Dysregulation and Functional Impairment in CD8+ T Cell ImmunityIn addition to immediate cell lethality, radiation exposure has longer term effects on the immune system, particularly T lymphocytes, causing persistent functional abnormalities post-exposure. Uncertainties remain regarding the long-lasting consequences on T cell immunity in acute-radiation-syndrome survivors and the underlying mechanisms. Here, we investigated delayed effects of acute radiation exposure on CD8+ T cell immunity using the Listeria monocytogenes infection model. Impaired CD8+ T cells activation, reduced terminal effector formation upon infection, and exacerbated listeriosis were found in mice at four weeks post-irradiation with sublethal doses. To elucidate how radiation affects the functionality of CD8+ T cells at various differentiation stages, we performed single-cell transcriptomic profiling. Irradiation altered cluster distribution pre-infection and impaired terminal effector and memory precursor effector formation post-infection. Differential gene analysis highlights radiation-induced gene expression changes, including cytokines and mitochondrial proteins, in a cluster-specific manner post-infection. The study demonstrates dysfunction of newly replenished naïve T cells, which results in impaired CD8+ T cell immunity after irradiation.2026/06/30GSE329314GSM9701630GSM9701633GSM9701634GSM9701631GSM9701632GSM9701637GSM9701635GSM970163634290329314Mus musculus