GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE329nnn/GSE329318/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE329318GEOGSEfalseEffect of interferon-gamma stimulation on the transcriptome of RANKL/M-CSF-induced osteoclastsInterferon-gamma (IFN-γ) is a pleiotropic cytokine with context-dependent effects on bone homeostasis, capable of both inhibiting and promoting osteoclastogenesis depending on the cellular milieu and stage of differentiation. To delineate the direct transcriptional consequences of IFN-γ exposure during terminal osteoclast differentiation, we performed RNA sequencing on RANKL/M-CSF-induced osteoclasts treated with or without IFN-γ. Differential expression analysis identified a suite of IFN-γ-responsive genes and revealed significant perturbation of pathways governing osteoclast fusion and bone resorption. Notably, we identified Lipocalin-2 (Lcn2) as a key downstream effector whose expression is robustly induced by IFN-γ. These data elucidate the molecular mechanisms by which IFN-γ translates immune signals into pro-osteoclastogenic instructions, highlighting a specific transcriptional program that enhances osteoclast differentiation and may contribute to inflammatory bone loss.2026/05/02GSE329318GSM9701684GSM9701685GSM9701688GSM9701689GSM9701686GSM970168734328329318Mus musculus