{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE329nnn/GSE329536/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Macaca mulatta"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE329536"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"A single-cell transcriptomic atlas of rhesus macaque hematopoiesis reveals age-related myeloid bias and differentiation changes","description":"Aging is accompanied by profound functional declines in the hematopoietic system, including diminished regenerative capacity and a skewing toward the myeloid lineage. While murine models have provided critical insights into hematopoietic stem cell (HSC) aging, the evolutionary distance to humans limits their translational applicability. Non-human primates, such as rhesus macaques, offer a highly relevant model, yet a comprehensive single-cell transcriptomic map of their aging hematopoietic system has been lacking. Here, we present a high-resolution single-cell RNA sequencing atlas of rhesus macaque hematopoiesis, profiling whole bone marrow, peripheral blood, and CD34-enriched progenitor cells from young and late-middle-aged cohorts. Our analysis delineates the transcriptional landscape of macaque hematopoiesis and identifies robust surface markers for prospective isolation of progenitor subpopulations. We uncover distinct, lineage-specific aging signatures, with the most profound transcriptional alterations occurring in multipotent progenitors and classical monocytes. Pseudotime and RNA velocity analyses reveal that aged HSCs reside in a more advanced state of differentiation with altered kinetics, providing a molecular basis for the observed age-related myeloid bias. Furthermore, cross-species integration with healthy human bone marrow data demonstrates profound transcriptional conservation of the stem and progenitor compartments between R. macaques and humans, with minimal species-specific divergence. These findings establish the rhesus macaque as a highly faithful surrogate for human hematopoietic aging and provide a critical resource for developing targeted interventions to rejuvenate the aging blood system.","dates":{"publication":"2026/09/16"},"accession":"GSE329536","cross_references":{"GSM":["GSM9705960","GSM9705920","GSM9705964","GSM9705963","GSM9705962","GSM9705961","GSM9705968","GSM9705924","GSM9705923","GSM9705967","GSM9705922","GSM9705966","GSM9705965","GSM9705921","GSM9705928","GSM9705927","GSM9705926","GSM9705925","GSM9705969","GSM9705929","GSM9705953","GSM9705952","GSM9705951","GSM9705950","GSM9705957","GSM9705956","GSM9705955","GSM9705954","GSM9705917","GSM9705916","GSM9705959","GSM9705958","GSM9705919","GSM9705918","GSM9705942","GSM9705941","GSM9705940","GSM9705946","GSM9705945","GSM9705944","GSM9705943","GSM9705949","GSM9705948","GSM9705947","GSM9705971","GSM9705970","GSM9705931","GSM9705930","GSM9705974","GSM9705973","GSM9705972","GSM9705935","GSM9705934","GSM9705933","GSM9705932","GSM9705939","GSM9705938","GSM9705937","GSM9705936"],"GPL":["27943"],"GSE":["329536"],"taxon":["Macaca mulatta"],"PMID":["[42618695]"]}}