GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE329nnn/GSE329609/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE329609GEOGSEfalseMouse embryo transcriptomes after two-cell blastomere separationThis study profiled mouse preimplantation embryos by Smart-seq2 RNA-seq to assess whether separation of late two-cell blastomeres alters the developmental transcriptomic program. The dataset includes control 8-cell embryos, split morula-stage embryos derived from two-cell blastomeres, control morula embryos, split blastocyst-stage embryos derived from two-cell blastomeres, and control blastocysts. The data were used to compare global transcriptomic similarity, developmental-stage effects, sample correlations, hierarchical clustering and pseudotime trajectories between split embryos and intact controls.2026/05/05GSE329609GSM9707480GSM9707481GSM9707470GSM9707475GSM9707486GSM9707487GSM9707476GSM9707465GSM9707488GSM9707477GSM9707466GSM9707478GSM9707467GSM9707482GSM9707471GSM9707472GSM9707483GSM9707484GSM9707473GSM9707485GSM9707474GSM9707479GSM9707468GSM970746924247329609Mus musculus