{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE329nnn/GSE329778/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"species":["Mus musculus"],"gds_type":["Genome binding/occupancy profiling by high throughput sequencing"," Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE329778"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Inflammatory memory primes the intestine for enhanced regenerative potential","description":"Intestinal regeneration after injury can occur through the expansion of surviving intestinal stem cells (ISCs) or via dedifferentiation of progenitor cells. To assess whether Lgr5⁺ ISCs that persist after irradiation—and their progeny—undergo long-term transcriptomic and epigenetic alterations, we performed single-cell multiome sequencing (scRNA-seq and scATAC-seq) using the 10x Genomics Chromium platform.Moreover,to investigate whether this dedifferentiation process leads to lasting transcriptomic and epigenetic changes, we used Krt20CreERT2:Rosa26-tdTomato mice to specifically label villus epithelial cells. In non-irradiated conditions, these cells are rapidly shed and lost. However, following irradiation, a subset of labeled cells dedifferentiates, migrates into the crypt compartment, and contributes to ISC regeneration. To characterize long-term molecular changes in these dedifferentiated cells and their progeny, we performed single-cell multiome sequencing (scRNA-seq and scATAC-seq) using the 10x Genomics Chromium platform.","dates":{"publication":"2026/09/16"},"accession":"GSE329778","cross_references":{"GSM":["GSM9711144","GSM9711145","GSM9711146","GSM9711147","GSM9711148","GSM9711149"],"GPL":["24247"],"GSE":["329778"],"taxon":["Mus musculus"]}}