{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE329nnn/GSE329836/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE329836"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Intermolecular 3′UTR-3′UTR interactions drive Wnt gene activation through heteromeric protein assembly","description":"Stem cell differentiation depends on transcription factors that are often encoded by mRNAs with highly conserved 3′UTRs. To determine their functional roles, we performed 3′UTR loss-of-function studies. Partial deletion of endogenous 3′UTRs altered stem cell differentiation efficiency in 7/10 cases. As 6/7 3′UTR deletions did not affect expression level of the encoded proteins, we reveal widespread abundance-independent regulatory roles of 3′UTRs. For example, 3′UTR deletion of CTNNB1, an mRNA that encodes the essential Wnt co-activator β-catenin, keeps β-catenin levels unaffected but impairs zebrafish embryogenesis and induction of the Wnt transcriptional program during human stem cell differentiation. We show that long intermolecular 3′UTR-3′UTR interactions between Wnt transcription factor mRNAs and CTNNB1 enable co-translational protein complex assembly of these transcription factors with β-catenin. As antisense oligonucleotide-mediated blocking of 3′UTR interactions impairs Wnt program induction, our findings indicate that transcriptional regulators can form functional units during protein biogenesis to be fully active.","dates":{"publication":"2026/05/05"},"accession":"GSE329836","cross_references":{"GSM":["GSM9712198","GSM9712176","GSM9712177","GSM9712199","GSM9712178","GSM9712179","GSM9712190","GSM9712191","GSM9712192","GSM9712193","GSM9712194","GSM9712195","GSM9712173","GSM9712174","GSM9712196","GSM9712197","GSM9712175","GSM9712187","GSM9712188","GSM9712200","GSM9712189","GSM9712201","GSM9712180","GSM9712181","GSM9712182","GSM9712183","GSM9712184","GSM9712185","GSM9712186"],"GPL":["34284"],"GSE":["329836"],"taxon":["Homo sapiens"]}}