{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE329nnn/GSE329992/"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE329992"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Autocrine PD-1-Blocking Nanobodies Enhance the Antitumor Efficacy of TCR-Like CAR-T Cells by Attenuating T Cell Exhaustion","description":"This study investigates a next-generation TCR-mimic (TCRm) chimeric antigen receptor T (CAR-T) cell platform engineered to overcome two major obstacles in solid tumor immunotherapy: intracellular tumor-associated antigen targeting and PD-1/PD-L1-mediated T cell exhaustion. We developed WT1-CAR-Nb T cells, which co-express a TCRm CAR specific for the WT1 peptide/HLA-A*02:01 complex and a constitutively secreted high-affinity anti-PD-1 nanobody. The transcriptomic dataset submitted here was generated to characterize the molecular signatures associated with enhanced functional durability conferred by autocrine PD-1 blockade. Specifically, RNA sequencing was performed on WT1-CAR-Nb T cells versus conventional WT1-CAR T cells following chronic antigen stimulation in vitro, aiming to delineate the differentially expressed genes and enriched pathways underpinning the attenuated exhaustion phenotype, preserved stem-like memory properties, and pro-survival transcriptional program of WT1-CAR-Nb T cells.","dates":{"publication":"2026/09/23"},"accession":"GSE329992","cross_references":{"GSM":["GSM9714803","GSM9714802","GSM9714805","GSM9714804"],"GPL":["16791"],"GSE":["329992"],"taxon":["Homo sapiens"],"PMID":["[42745268]"]}}