GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE329nnn/GSE329992/primaryOK200TranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE329992GEOGSEfalseAutocrine PD-1-Blocking Nanobodies Enhance the Antitumor Efficacy of TCR-Like CAR-T Cells by Attenuating T Cell ExhaustionThis study investigates a next-generation TCR-mimic (TCRm) chimeric antigen receptor T (CAR-T) cell platform engineered to overcome two major obstacles in solid tumor immunotherapy: intracellular tumor-associated antigen targeting and PD-1/PD-L1-mediated T cell exhaustion. We developed WT1-CAR-Nb T cells, which co-express a TCRm CAR specific for the WT1 peptide/HLA-A*02:01 complex and a constitutively secreted high-affinity anti-PD-1 nanobody. The transcriptomic dataset submitted here was generated to characterize the molecular signatures associated with enhanced functional durability conferred by autocrine PD-1 blockade. Specifically, RNA sequencing was performed on WT1-CAR-Nb T cells versus conventional WT1-CAR T cells following chronic antigen stimulation in vitro, aiming to delineate the differentially expressed genes and enriched pathways underpinning the attenuated exhaustion phenotype, preserved stem-like memory properties, and pro-survival transcriptional program of WT1-CAR-Nb T cells.2026/09/23GSE329992GSM9714803GSM9714802GSM9714805GSM971480416791329992Homo sapiens[42745268]