GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE330nnn/GSE330079/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE330079GEOGSEfalseBulk RNA sequencing of control (NT) and Spp1 knockout mouse samplesBulk RNA sequencing was performed to assess transcriptional differences between control (NT) and Spp1 knockout (KO) samples. RNA libraries were sequenced using paired-end Illumina sequencing. Reads were trimmed, aligned to the mouse genome (mm10), and quantified to generate gene-level count matrices. Differential expression analysis was performed to identify transcriptional changes associated with gene knockout.2026/07/28GSE330079GSM9717579GSM9717577GSM9717578GSM9717580GSM9717581GSM971757624247330079Mus musculus