{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE330nnn/GSE330085/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE330085"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Sex-divergent skeletal muscle remodelling emerges early in cancer cachexia","description":"Cancer cachexia is often studied after overt weight and muscle loss are established, limiting insight into early initiating mechanisms and sex-dependent trajectories. This dataset profiles pre-cachectic skeletal muscle transcriptional remodelling in a stage-resolved Colon-26 (C26) model in male and female BALB/c mice. Tibialis anterior muscle was collected at the pre-cachectic stage (tumour palpable, prior to overt hindlimb muscle mass loss) from tumour-bearing mice and sex-matched sham controls (n=4/group; 16 samples total). Poly(A)-selected stranded bulk RNA sequencing (150 bp paired-end; Illumina NovaSeq X Plus) was performed to identify early tumour-associated gene expression changes and sexually dimorphic responses. Reads were aligned to the Mus musculus reference genome (mm39/GRCm39) and gene-level counts were generated for differential expression analysis. Differential expression was assessed using edgeR with TMM normalisation and false discovery rate (FDR) < 0.05. The dataset reveals extensive pre-cachectic transcriptional reprogramming with strong sex specificity, including early regulation of proteostasis, innate immune, extracellular matrix, and metabolic/insulin-related pathways. This resource supports interrogation of early, sex-divergent skeletal muscle adaptations during cachexia initiation and identification of candidate biomarkers and therapeutic targets before overt wasting is established.","dates":{"publication":"2026/08/24"},"accession":"GSE330085","cross_references":{"GSM":["GSM9717711","GSM9717700","GSM9717701","GSM9717712","GSM9717698","GSM9717710","GSM9717699","GSM9717704","GSM9717705","GSM9717702","GSM9717703","GSM9717708","GSM9717709","GSM9717706","GSM9717707","GSM9717697"],"GPL":["34290"],"GSE":["330085"],"taxon":["Mus musculus"],"PMID":["[42463245]"]}}