GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE330nnn/GSE330085/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE330085GEOGSEfalseSex-divergent skeletal muscle remodelling emerges early in cancer cachexiaCancer cachexia is often studied after overt weight and muscle loss are established, limiting insight into early initiating mechanisms and sex-dependent trajectories. This dataset profiles pre-cachectic skeletal muscle transcriptional remodelling in a stage-resolved Colon-26 (C26) model in male and female BALB/c mice. Tibialis anterior muscle was collected at the pre-cachectic stage (tumour palpable, prior to overt hindlimb muscle mass loss) from tumour-bearing mice and sex-matched sham controls (n=4/group; 16 samples total). Poly(A)-selected stranded bulk RNA sequencing (150 bp paired-end; Illumina NovaSeq X Plus) was performed to identify early tumour-associated gene expression changes and sexually dimorphic responses. Reads were aligned to the Mus musculus reference genome (mm39/GRCm39) and gene-level counts were generated for differential expression analysis. Differential expression was assessed using edgeR with TMM normalisation and false discovery rate (FDR) < 0.05. The dataset reveals extensive pre-cachectic transcriptional reprogramming with strong sex specificity, including early regulation of proteostasis, innate immune, extracellular matrix, and metabolic/insulin-related pathways. This resource supports interrogation of early, sex-divergent skeletal muscle adaptations during cachexia initiation and identification of candidate biomarkers and therapeutic targets before overt wasting is established.2026/08/24GSE330085GSM9717711GSM9717700GSM9717701GSM9717712GSM9717698GSM9717710GSM9717699GSM9717704GSM9717705GSM9717702GSM9717703GSM9717708GSM9717709GSM9717706GSM9717707GSM971769734290330085Mus musculus[42463245]