GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE330nnn/GSE330134/primaryOK200OtherHomo sapiensOtherhttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE330134GEOGSEfalseMassively Multiplexed Sequencing-Based Host Cell Reactivation Assay Profiles DNA Repair at Single-Nucleotide and -Cell Resolution [amplicon]Defects in DNA repair are clinical biomarkers for therapeutic vulnerabilities in multiple cancer types. However, the characterization of DNA repair in cancer remains bottlenecked by a lack of reliable genomic predictors and the low throughput of existing functional assays. Here, we present mmHCR-seq, a sequencing-based and massively multiplexed Host Cell Reactivation assay that enables parallel quantification of functional repair efficiency and accuracy across all major repair pathways via RNA-seq readout. We demonstrate that mmHCR-seq can detect deficiencies and measure kinetics of nine DNA repair pathways simultaneously. The single-nucleotide resolution revealed lesion-dependent kinetics for base excision repair intermediates. By integrating mmHCR-seq with single-cell RNA-seq, we mapped repair capacity in a 19-cell line pool at cellular resolution and coupled repair phenotypes to expression states. Therein, cell cycle effects were unraveled without the need for synchronization. Overall, mmHCR-seq provides a scalable, high-resolution platform for dissecting DNA repair mechanisms and identifying new repair-associated vulnerabilities in cancer.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 sapiens