GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE330nnn/GSE330356/primaryOK200TranscriptomicsPyricularia oryzaeExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE330356GEOGSEfalseThe secreted protein MoSun4 of Magnaporthe oryzae regulates pathogenicity by repressing the expression of virulence-related genesThe SUN family protein MoSun4 in Magnaporthe oryzae has been previously implicated in mitophagy and can be used as a target for reducing rice blast, but its role as a secreted protein remain poorly understood. In this study, signal peptide prediction and yeast secretion assays confirmed the activity of MoSun4 signal peptide, and co-localization revealed its extra-invasive hyphal membrane (EIHM) localization, establishing MoSun4 as a secreted protein. In addition, deletion of MoSUN4 severely attenuated the hyphal growth, conidiation and virulence of M. oryzae. We further showed that MoSun4 represses the expression of virulence-related genes, including multiple genes involved in cell wall degradation (eglC, eglD), secondary metabolism (gliK), and melanin biosynthesis (SDH1, BUF1, Cmr1, ALB1). Collectively, our study reveals that MoSun4 is a secreted protein and regulates pathogenicity by repressing the expression of virulence-related genes, providing new insights into the function of SUN family proteins.2026/05/20GSE330356GSM9724341GSM9724340GSM9724339GSM9724344GSM9724343GSM972434236936330356Pyricularia oryzae