{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE330nnn/GSE330471/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE330471"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Single-cell transcriptomic profiling reveals that KLF2 limits tumor cell plasticity in hepatocellular carcinoma","description":"Hepatocellular carcinoma (HCC) is a highly heterogeneous primary liver malignancy whose malignant progression is strongly associated with tumor cell plasticity. In epithelial-derived liver cancer, epithelial-mesenchymal plasticity (EMP), including epithelial-mesenchymal transition (EMT) and partial EMT (pEMT) programs, is considered a key driver of tumor progression, therapeutic resistance, recurrence, and metastasis. KLF2 has been suggested to function as a tumor suppressor in liver cancer, but its role in regulating tumor cell plasticity in HCC remains incompletely understood. To address this question, we established a c-MYC/AKT-driven murine HCC model with or without KLF2 overexpression and performed single-cell RNA sequencing on tumor tissues. Mouse HCC was induced by hydrodynamic tail vein injection of pT3-EF1α-c-MYC and pT3-EF1α-AKT plasmids together with the SB100X transposase plasmid, and liver tumors developed 10 weeks after injection. In the KLF2 overexpression group, pT3-EF1α-KLF2 was additionally co-injected. Single-cell RNA sequencing was performed using the BGI C4 platform. This dataset provides a resource for characterizing tumor cell plasticity programs and KLF2-associated transcriptional changes in HCC at single-cell resolution.","dates":{"publication":"2026/05/16"},"accession":"GSE330471","cross_references":{"GSM":["GSM9727339","GSM9727340","GSM9727341","GSM9727342"],"GPL":["28330"],"GSE":["330471"],"taxon":["Mus musculus"]}}