{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE330nnn/GSE330527/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Pteromalus puparum"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE330527"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Full length transcriptome sequencing of salivary glands of Pteromalus puparum","description":"Animal saliva provides an excellent model for studying adaptive evolution, yet the functional significance of alternative mRNA isoforms in parasitoid salivary systems remains poorly understood. Here, using integrative full-length transcript sequencing and expression profiling, we generated an isoform-resolved transcriptomic landscape of salivary genes in the endoparasitoid wasp Pteromalus puparum. We identified 133 high-confidence salivary genes, more than 75% of which produced multiple transcript isoforms. Proteomic analyses confirmed that alternative splicing directly contributes to salivary protein diversity, with eight genes encoding distinct protein isoforms. Twelve salivary genes exhibited gland-biased isoform usage, including PpSerpin3, whose short isoform was specifically expressed in salivary and venom glands and suppressed host melanization. Comparative multi-omics analyses further revealed that salivary and venom systems share a conserved genetic core but achieve functional specialization through tissue-specific gene family co-option and extensive isoform switching. Together, these findings establish an isoform-resolved framework for the parasitoid salivary system and demonstrate that alternative splicing promotes salivary protein diversification and gland-specific functional evolution.","dates":{"publication":"2026/05/13"},"accession":"GSE330527","cross_references":{"GSM":["GSM9728214","GSM9728206","GSM9728208","GSM9728207","GSM9728209","GSM9728211","GSM9728210","GSM9728213","GSM9728212"],"GPL":["36946"],"GSE":["330527"],"taxon":["Pteromalus puparum"]}}