{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE330nnn/GSE330533/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE330533"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"NGFR-marked basal duct progenitors drive ductal-acinar regeneration in injured salivary glands","description":"Severe salivary gland injury causes long-term structural and functional impairment, yet the epithelial populations associated with repair remain incompletely defined. Here, we identify NGFR as a surface marker of a basal duct-associated epithelial population with organoid-forming capacity in the human salivary gland and show that Ngfr marks a conserved injury-responsive epithelial population in the mouse salivary gland. Single-cell RNA sequencing of human salivary gland tissue revealed that NGFR was enriched in a restricted basal duct subpopulation distinct from differentiated epithelial and myoepithelial compartments. NGFR-positive cells exhibited a progenitor-like transcriptional profile and were enriched at early pseudotime positions in inferred ductal-acinar differentiation trajectories. Functionally, NGFR-positive cells isolated from human tissue and organoids showed enhanced organoid-forming capacity, and NGFR-enriched organoids engrafted into injured mouse salivary glands. In mouse salivary glands, Ngfr marked a rare epithelial population whose isolated Ngfr-positive fraction showed enriched organoid-forming activity and was detected in injury-associated ductal regions after duct ligation and local inflammatory injury. Lineage tracing using Ngfr-CreERT2; Rosa26-tdTomato mice further showed that Ngfr-lineage cells contribute to ductal and acinar epithelial compartments during post-injury regeneration. Together, these findings define NGFR in human and Ngfr in mouse as conserved markers of an injury-responsive basal duct epithelial population that can be prospectively isolated and contributes to ductal and acinar epithelial compartments during post-injury salivary gland regeneration.","dates":{"publication":"2026/05/15"},"accession":"GSE330533","cross_references":{"GSM":["GSM9728240","GSM9728241"],"GPL":["24676"],"GSE":["330533"],"taxon":["Homo sapiens"]}}