GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE330nnn/GSE330696/primaryOK200TranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE330696GEOGSEfalseBulk RNA-seq of human colonic organoids treated with IFNγ and TNFHuman intestinal epithelial organoids derived from non-IBD patients were used to investigate transcriptional responses associated with inflammatory cytokine-induced epithelial cell death. Organoids were stimulated with IFNγ and TNF to model inflammatory injury observed in IBD. Bulk RNA sequencing was performed to characterise cytokine-driven gene expression programs associated with inducible nitric oxide synthase (iNOS) activation, epithelial stress responses, mitochondrial apoptosis, and inflammatory signalling pathways. Comparative transcriptomic analyses identified inflammatory and cell death-associated gene signatures induced by IFNγ/TNF treatment and revealed molecular pathways linked to epithelial injury in human intestinal organoids. These data provide a resource for understanding how inflammatory cytokines regulate epithelial cell death and transcriptional remodelling in the intestinal epithelium during IBD-associated inflammation.2026/09/03GSE330696GSM9730609GSM9730629GSM9730618GSM9730607GSM9730606GSM9730628GSM9730617GSM9730622GSM9730611GSM9730600GSM9730610GSM9730621GSM9730599GSM9730598GSM9730620GSM9730627GSM9730616GSM9730604GSM9730625GSM9730614GSM9730603GSM9730613GSM9730624GSM973060218573330696Homo sapiens[42658927]