GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE330nnn/GSE330702/primaryOK200TranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE330702GEOGSEfalseRNA-seq analysis of Nogo-B knockdown in human dermal lymphatic endothelial cells under TGF-β2 stimulationNogo-B (RTN4B), an endoplasmic reticulum-associated protein, plays a critical role in regulating endothelial cell function and vascular remodeling. However, its role in lymphatic endothelial cells (LECs) and in response to profibrotic signaling remains poorly understood. Transforming growth factor-β2 (TGF-β2) is a key regulator of endothelial phenotypes and has been implicated in lymphatic dysfunction under pathological conditions. In this study, we performed RNA sequencing (RNA-seq) to investigate the transcriptional changes associated with Nogo-B knockdown in human dermal lymphatic endothelial cells (HDLECs) under TGF-β2 stimulation. HDLECs were transduced with shRNA targeting Nogo-B (shNogo-B) or control shRNA (shGFP), followed by treatment with TGF-β2 for 72 hours. Total RNA was extracted and subjected to high-throughput sequencing. Comparative transcriptomic analysis was conducted to identify differentially expressed genes and pathways regulated by Nogo-B under TGF-β2-stimulated conditions. This dataset provides insights into the molecular mechanisms by which Nogo-B modulates lymphatic endothelial cell responses to TGF-β signaling, including pathways related to extracellular matrix organization, cell migration, proliferation, and cytoskeletal remodeling. These data serve as a resource for understanding the role of Nogo-B in lymphatic endothelial biology and its contribution to TGF-β-mediated signaling and dysfunction.2026/05/13GSE330702GSM9730777GSM9730776GSM9730775GSM9730774GSM9730779GSM9730778GSM9730780GSM9730773GSM9730772GSM9730782GSM9730771GSM973078124676330702Homo sapiens