GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE330nnn/GSE330728/primary200OKTranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE330728GEOGSEfalseSingle-nucleus RNA-seq atlas of 17 ASD-risk mouse lines under early-postnatal fluoxetine or lithium exposure (4-plex On-Chip Multiplexed)Autism spectrum disorder (ASD) involves more than 1,000 risk genes, complicating mechanistic subtyping. To resolve the cellular basis of transcriptomic stratification, we generated single-nucleus RNA-seq (snRNA-seq) profiles from the prefrontal cortex of 17 mouse lines carrying ASD-associated synaptic, chromatin-regulatory, and signaling mutations, together with C57BL/6J wild-type controls. Both sexes were profiled under baseline and early-postnatal (P3-P25) fluoxetine or lithium exposure. Four mice were multiplexed per 10x Genomics GEM-X v4 4-plex On-Chip Multiplexing (OCM) library, yielding 53 sequencing libraries (tubes) covering 212 individual mice. After downstream QC, approximately 1,016,895 nuclei from 205 mice were retained at postnatal day 25.2026/07/28GSE330728GSM9731290GSM9731291GSM9731292GSM9731293GSM9731298GSM9731331GSM9731299GSM9731294GSM9731295GSM9731296GSM9731330GSM9731297GSM9731306GSM9731307GSM9731308GSM9731309GSM9731302GSM9731303GSM9731304GSM9731305GSM9731300GSM9731301GSM9731317GSM9731318GSM9731319GSM9731313GSM9731314GSM9731315GSM9731316GSM9731310GSM9731311GSM9731312GSM9731279GSM9731280GSM9731281GSM9731282GSM9731328GSM9731329GSM9731324GSM9731325GSM9731326GSM9731327GSM9731320GSM9731287GSM9731321GSM9731288GSM9731289GSM9731322GSM9731323GSM9731283GSM9731284GSM9731285GSM973128634328330728Mus musculus