{"database":"GEO","file_versions":[],"scores":null,"additional":{"omics_type":["Genomics"],"species":["Neurospora crassa"],"gds_type":["Genome binding/occupancy profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE330894"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"RTT109 regulates Polycomb-repressed genes independently from H3K56 acetylation status","description":"In animals, plants, and some fungi, Polycomb Repressive Complex 2 (PRC2) catalyzes trimethylation of histone H3 lysine 27 (H3K27me3) to establish transcriptionally repressed chromatin. Here, we identify the histone acetyltransferase RTT109 as a key regulator of PRC2-repressed domains in the model fungus Neurospora crassa. Although RTT109 interacts with the VPS75 homolog Nucleosome Assembly Factor 2 (NAF-2), we show that proper structure and function of PRC2-methylated chromatin require RTT109 catalytic activity but are independent of NAF-2 and H3K56 acetylation. We further demonstrate that H3K27me3 can be stably propagated over multiple rounds of mitosis in the absence of sequence-specific PRC2 targeting, and that RTT109 is essential for maintenance of the repressed state. These findings uncover a replication-linked mechanism for epigenetic memory and establish RTT109 as a key regulator of Polycomb-mediated chromatin inheritance.","dates":{"publication":"2026/05/17"},"accession":"GSE330894","cross_references":{"GSM":["GSM9735250","GSM9735142","GSM9735143","GSM9735141","GSM9735148","GSM9735149","GSM9735146","GSM9735147","GSM9735144","GSM9735145","GSM9735230","GSM9735197","GSM9735231","GSM9735198","GSM9735195","GSM9735196","GSM9735193","GSM9735194","GSM9735191","GSM9735192","GSM9735190","GSM9735238","GSM9735239","GSM9735236","GSM9735237","GSM9735234","GSM9735235","GSM9735199","GSM9735232","GSM9735233","GSM9735241","GSM9735242","GSM9735240","GSM9735249","GSM9735247","GSM9735248","GSM9735245","GSM9735246","GSM9735243","GSM9735244","GSM9735175","GSM9735176","GSM9735173","GSM9735174","GSM9735171","GSM9735172","GSM9735170","GSM9735218","GSM9735219","GSM9735216","GSM9735217","GSM9735214","GSM9735215","GSM9735179","GSM9735212","GSM9735213","GSM9735210","GSM9735177","GSM9735211","GSM9735178","GSM9735186","GSM9735187","GSM9735220","GSM9735184","GSM9735185","GSM9735182","GSM9735183","GSM9735180","GSM9735181","GSM9735229","GSM9735227","GSM9735228","GSM9735225","GSM9735226","GSM9735223","GSM9735224","GSM9735188","GSM9735221","GSM9735222","GSM9735189","GSM9735153","GSM9735154","GSM9735151","GSM9735152","GSM9735150","GSM9735159","GSM9735157","GSM9735158","GSM9735155","GSM9735156","GSM9735164","GSM9735165","GSM9735162","GSM9735163","GSM9735160","GSM9735161","GSM9735209","GSM9735207","GSM9735208","GSM9735205","GSM9735206","GSM9735203","GSM9735204","GSM9735168","GSM9735201","GSM9735202","GSM9735169","GSM9735166","GSM9735167","GSM9735200"],"GPL":["35294"],"GSE":["330894"],"taxon":["Neurospora crassa"]}}