GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE330nnn/GSE330894/primaryOK200GenomicsNeurospora crassaGenome binding/occupancy profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE330894GEOGSEfalseRTT109 regulates Polycomb-repressed genes independently from H3K56 acetylation statusIn animals, plants, and some fungi, Polycomb Repressive Complex 2 (PRC2) catalyzes trimethylation of histone H3 lysine 27 (H3K27me3) to establish transcriptionally repressed chromatin. Here, we identify the histone acetyltransferase RTT109 as a key regulator of PRC2-repressed domains in the model fungus Neurospora crassa. Although RTT109 interacts with the VPS75 homolog Nucleosome Assembly Factor 2 (NAF-2), we show that proper structure and function of PRC2-methylated chromatin require RTT109 catalytic activity but are independent of NAF-2 and H3K56 acetylation. We further demonstrate that H3K27me3 can be stably propagated over multiple rounds of mitosis in the absence of sequence-specific PRC2 targeting, and that RTT109 is essential for maintenance of the repressed state. These findings uncover a replication-linked mechanism for epigenetic memory and establish RTT109 as a key regulator of Polycomb-mediated chromatin inheritance.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 crassa