{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE330nnn/GSE330919/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE330919"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Effect of RSL3 and IFN-γ on gene expression in 526MEL cells","description":"Tumor masses often exhibit heterogeneity, including escape variant clones that lack antigen-presenting machinery and/or tumor antigens, which poses a major challenge to immunotherapy. Ferroptosis, a form of regulated cell death driven by iron-dependent lipid peroxidation, has been shown to effectively induce cell death in various tumor cells. Recent studies have reported that IFN-γ suppresses the expression of System Xc-, thereby enhancing the induction of ferroptosis. Based on this, we hypothesized that combining immunotherapy with ferroptosis inducers could enhance antitumor effects against both antigen-positive and antigen-negative tumor cells. Consistent with this hypothesis, we comprehensively analyzed mRNA expression changes in 526MEL cells treated with the combination of RSL3 and IFN-γ using RNA sequencing. Our data suggest that multiple ferroptosis-related pathways, including the CoQ-dependent membrane repair pathway (FSP1–CoQ axis), the DHODH–CoQ mitochondrial redox pathway (DHODH–CoQ axis), the CYB5R–cytochrome b5 lipid redox pathway (CYB5R axis), and the thioredoxin–peroxiredoxin repair pathway (PRDX/TXN axis), may contribute to the synergistic effects of combined RSL3 and IFN-γ treatment in human melanoma cell lines.","dates":{"publication":"2026/06/16"},"accession":"GSE330919","cross_references":{"GSM":["GSM9735739","GSM9735737","GSM9735738","GSM9735735","GSM9735736","GSM9735744","GSM9735745","GSM9735734","GSM9735742","GSM9735743","GSM9735740","GSM9735741"],"GPL":["34284"],"GSE":["330919"],"taxon":["Homo sapiens"]}}