GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE331nnn/GSE331007/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE331007GEOGSEfalseEffects of T cell–specific IL-21 isoform expression on gene expression in mammary epithelial cells.Interleukin-21 (IL-21) is a pleiotropic cytokine produced by CD4⁺ T cells that critically regulates the differentiation and functions of various adaptive and innate immune cells. In this study, to investigate the chronic effects of IL-21 on gene expression in mammary epithelial cells (MECs), MECs were isolated from 10-week-old transgenic mice expressing a membrane-bound IL-21 isoform specifically in T cells (IL-21isoTg) and subjected to RNA sequencing analysis, followed by comparison with wild-type controls. IL-21isoTg MECs showed marked upregulation of genes associated with mammary gland development and lactation and Spp1.2026/05/18GSE331007GSM9737277GSM9737276GSM9737275GSM9737280GSM9737279GSM973727824247331007Mus musculus