{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE331nnn/GSE331042/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":[" Other","Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE331042"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Mechanoimmunological Control of Metastatic Site Selection","description":"To explore the molecular mechanisms underlying stiffness modulation in vivo, GFP+ B16F10 MTCs from the lungs of wild type mice and from the lungs and bones of Prf1-/- mice were subjected to single cell RNA-sequencing (scRNA-seq). Uniform manifold approximation and projection (UMAP) analysis of the resulting data revealed 9 distinguishable populations of cancer cells. Lung metastases from wild type and Prf1-/- animals contained all 9 populations, implying that cellular cytotoxicity does not drastically alter tumor composition in this organ. Nevertheless, subtle shifts in the size and make-up of certain clusters were apparent, consistent with some degree of immune pressure. B16F10 composition differed dramatically in Prf1-/- bone, with several clusters shifting substantially in the UMAP plot or disappearing altogether.","dates":{"publication":"2026/07/28"},"accession":"GSE331042","cross_references":{"GSM":["GSM9738019","GSM9738018"],"GPL":["24247"],"GSE":["331042"],"taxon":["Mus musculus"]}}