GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE331nnn/GSE331042/primary200OKTranscriptomicsMus musculus OtherExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE331042GEOGSEfalseMechanoimmunological Control of Metastatic Site SelectionTo explore the molecular mechanisms underlying stiffness modulation in vivo, GFP+ B16F10 MTCs from the lungs of wild type mice and from the lungs and bones of Prf1-/- mice were subjected to single cell RNA-sequencing (scRNA-seq). Uniform manifold approximation and projection (UMAP) analysis of the resulting data revealed 9 distinguishable populations of cancer cells. Lung metastases from wild type and Prf1-/- animals contained all 9 populations, implying that cellular cytotoxicity does not drastically alter tumor composition in this organ. Nevertheless, subtle shifts in the size and make-up of certain clusters were apparent, consistent with some degree of immune pressure. B16F10 composition differed dramatically in Prf1-/- bone, with several clusters shifting substantially in the UMAP plot or disappearing altogether.2026/07/28GSE331042GSM9738019GSM973801824247331042Mus musculus