GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE331nnn/GSE331107/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE331107GEOGSEfalseThe transcription factor AFF3 is necessary to maintain metabolic quiescence in naïve CD8 T cells and prevent premature immune agingNaïve CD8 T cells must be maintained in a quiescent metabolic state in order to respond robustly to infection while avoiding inappropriate activation and causing immunopathology. With age this quiescent state is lost and the CD8 T cell response to infection decreases. The factors regulating metabolic quiescence of CD8 T cells and how this regulation is lost during aging are not completely understood. Herein, we identify the transcription factor AFF3 as a novel regulator of metabolic quiescence in naïve CD8 T cells. While naïve AFF3 deficient CD8 T cells are more metabolically active prior to infection, they have reduced accumulation in response to viral infection and this is correlated with a poor capacity to engage glycolysis. During aging in both murine and human CD8 T cells, AFF3 expression is reducing, correlating with to a loss of quiescent metabolism without the cells entering an activated state. Our data highlights the role of the metabolism in CD8 T cell quiescence and identifies a novel transcription factor that may be a target to reinvigorate CD8 T cell responses during aging.2026/06/01GSE331107GSM9739114GSM9739136GSM9739135GSM9739113GSM9739134GSM9739112GSM9739111GSM9739133GSM9739132GSM9739110GSM9739131GSM9739130GSM9739119GSM9739118GSM9739117GSM9739116GSM9739115GSM9739125GSM9739124GSM9739123GSM9739122GSM9739121GSM9739120GSM9739109GSM9739108GSM9739129GSM9739107GSM9739128GSM9739127GSM973912634290331107Mus musculus