{"database":"GEO","file_versions":[],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by array"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE331111"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Expression data from OT-I CD8 T cells that are either naïve or isolated on day 5 following immunization with DC-OVA or with DC-OVA + LCMV","description":"Inflamatory cytokines have a number of effects on the differentiation and function of CD8 T cells. To separate the effects of TCR/co-stimulation from the effects of inflammatory cytokines, we took an immunization approach. We transferred 1 x 10^4 OT-I CD8 T cells to naive C57BL/6 mice on day -1. On day 0 mice were immunized with either 5 x 10^5 OVA peptide loaded, LPS-matured dendritic cells (DC-OVA) injected ip or 5 x 10^5 DC-OVA and 2 x 10^5 pfu of LCMV injected ip. On day 5 following the indicated treatment, OT-I CD8 T cells were enriched via AutoMACS and sorted via flow cytometry prior to RNA extraction from 1 x 10^6 cells using an RNeasy kit (Qiagen). Naive OT-I CD8 T cells were enriched and sorted for RNA extraction to serve as a control. Each group had 3 biological replicates.","dates":{"publication":"2026/06/01"},"accession":"GSE331111","cross_references":{"GSM":["GSM9739179","GSM9739178","GSM9739177","GSM9739176","GSM9739184","GSM9739183","GSM9739182","GSM9739181","GSM9739180"],"GPL":["6096"],"GSE":["331111"],"taxon":["Mus musculus"]}}