{"database":"GEO","file_versions":[],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE331144"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Transcriptional and spatial profiling of fibroblasts from human lungs highlights CTHRC1+ cells as fibrogenic signaling hubs in fibrosis","description":"This dataset contains single-cell RNA sequencing (scRNA-seq) data from native human lung tissue obtained from idiopathic pulmonary fibrosis (IPF) patients and age-matched controls. Samples were collected from IPF upper lobe, IPF lower lobe, and age-matched control lung tissue and processed using the 10x Genomics Chromium platform. Unsupervised clustering identified six transcriptionally distinct mesenchymal subtypes — alveolar, adventitial, inflammatory, CTHRC1⁺, peribronchial and smooth muscle cell — across conditions. Differential gene expression, pathway enrichment, and cell-cell communication analyses were performed to characterize subtype-specific transcriptional programs, ECM remodeling signatures, and intercellular signaling networks in the fibrotic lung niche. CTHRC1⁺ fibroblasts emerged as a dominant fibrogenic hub in IPF, characterized by elevated fibrillar collagen expression, broad outgoing signaling through collagen, fibronectin, and periostin pathways, and spatial co-localization with fibroblastic foci. This dataset provides a transcriptional resource for investigating mesenchymal heterogeneity and fibrogenic cell states in human pulmonary fibrosis.","dates":{"publication":"2026/05/29"},"accession":"GSE331144","cross_references":{"GSM":["GSM9739687","GSM9739688","GSM9739689","GSM9739690","GSM9739691","GSM9739692","GSM9739693","GSM9739694","GSM9739695"],"GPL":["24676"],"GSE":["331144"],"taxon":["Homo sapiens"]}}