GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE331nnn/GSE331223/primaryOK200TranscriptomicsCaenorhabditis elegansExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE331223GEOGSEfalseΔlpp Mutant E. coli Reduces Lipid Content in Caenorhabditis elegans via Phosphatidylglycerol-Mediated Inhibition of Fatty Acid BiosynthesisIn this study, we integrated in vivo lipid visualization, whole-transcriptome RNA sequencing, and lipidomics to investigate the effects of the Δlpp mutant of Escherichia coli (E. coli) on Caenorhabditis elegans (C. elegans). Our results demonstrated that the Δlpp mutant significantly reduced fat content across three C. elegans strains. Transcriptome analysis revealed substantial alterations in the expression of genes associated with fatty acid metabolism, particularly those involved in fatty acid biosynthesis, which were notably downregulated. Lipidomic profiling confirmed an upregulation of phosphatidylglycerol (PG) and indicated its potential involvement in regulating fatty acid biosynthesis. Importantly, PG supplementation significantly decreased lipid accumulation in C. elegans. Furthermore, we identified and validated four downregulated genes whose reduced expression was associated with lower lipid levels. These findings underscore the therapeutic potential of engineered probiotics in lipid-related metabolic disorders and offer novel mechanistic insights into how genetic variations in gut microbes influence host metabolic health.2026/05/22GSE331223GSM9742238GSM9742239GSM9742236GSM9742237GSM9742229GSM9742230GSM9742231GSM9742240GSM9742234GSM9742235GSM9742232GSM974223326672331223Caenorhabditis elegans