GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE331nnn/GSE331301/primaryOK200GenomicsHomo sapiensGenome binding/occupancy profiling by high throughput sequencing Expression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE331301GEOGSEfalseDistal Enhancer-Insulator Module of GDF6 is Essential for Cochlear Formation [single-cell multiome]Mutations in approximately 30 genes have been linked to cochlear malformations, but the contribution of noncoding regulatory elements remains largely unclear. This study investigates the function of distal enhancer elements in the transcriptional regulation of GDF6, a gene implicated in cochlear development. Using mouse models with targeted deletions, human inner ear organoids, and CRISPR interference (CRISPRi), we identified a downstream regulatory interval harboring a developmental enhancer required to maintain GDF6 expression during otic epithelial maturation and cochlear morphogenesis. To characterize cellular composition and chromatin accessibility changes in patient (166-101) versus control (CA-26) iPSC-derived otic progenitor cells, we performed joint single-nucleus RNA-seq and ATAC-seq (10X Genomics Multiome) on CD271+ enriched populations.2026/06/05GSE331301GSM9743603GSM9743606GSM9743605GSM974360424676331301Homo sapiens