{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE331nnn/GSE331341/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE331341"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"A Cost-Effective Nuclei Isolation Protocol for Single Nucleus RNA-Sequencing of Mouse HFpEF Hearts","description":"Single cell or single nuclei RNA-sequencing (snRNAseq) enables high-resolution profiling of cellular states, but sample preparation is often costly and complicated by debris formation and cell clumping. We describe a cost-effective approach using commercially available buffers and standard laboratory equipment to isolate high-quality nuclei from diseased cardiac tissue for reproducible downstream snRNAseq analysis. In addition, we include a previously unpublished heart failure with preserved ejection fraction (HFpEF) vs Chow dataset generated using this protocol.","dates":{"publication":"2026/09/12"},"accession":"GSE331341","cross_references":{"GSM":["GSM9744255","GSM9744254","GSM9744259","GSM9744258","GSM9744257","GSM9744256"],"GPL":["34328"],"GSE":["331341"],"taxon":["Mus musculus"]}}