GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE331nnn/GSE331341/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE331341GEOGSEfalseA Cost-Effective Nuclei Isolation Protocol for Single Nucleus RNA-Sequencing of Mouse HFpEF HeartsSingle cell or single nuclei RNA-sequencing (snRNAseq) enables high-resolution profiling of cellular states, but sample preparation is often costly and complicated by debris formation and cell clumping. We describe a cost-effective approach using commercially available buffers and standard laboratory equipment to isolate high-quality nuclei from diseased cardiac tissue for reproducible downstream snRNAseq analysis. In addition, we include a previously unpublished heart failure with preserved ejection fraction (HFpEF) vs Chow dataset generated using this protocol.2026/09/12GSE331341GSM9744255GSM9744254GSM9744259GSM9744258GSM9744257GSM974425634328331341Mus musculus