{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE331nnn/GSE331378/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":[" Mus musculus","Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE331378"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Bulk RNA-seq of PC3-derived human prostate cancer xenografts and matched mouse blood following treatment with the bivalent BET bromodomain inhibitor MS645 or the monovalent inhibitor JQ1","description":"Male ICR SCID mice (5-6 weeks old) were implanted subcutaneously with 5 x 10^6 PC3 cells. When tumors reached ~200 mm^3, mice were randomized to one of three treatment arms: vehicle, JQ1 (30 mg/kg), or MS645 (20 mg/kg). Compounds were administered intraperitoneally. At study end, mice were sacrificed and matched xenograft tumor and whole-blood specimens were collected and snap-frozen for bulk RNA-seq. Tumor RNA-seq reads were aligned to the human reference genome (GRCh38/hg38) and blood RNA-seq reads to the mouse reference genome (GRCm38/mm10). Differential expression analysis (DESeq2) was used to compare MS645 vs vehicle and JQ1 vs vehicle in each compartment. The study identifies the BRD4-RUVBL1/2 axis as a mediator of intrinsic resistance to monovalent BET bromodomain inhibition in AR-negative prostate cancer and demonstrates that bivalent BET targeting by MS645 reprograms resistant tumors toward a treatment-responsive transcriptional state.","dates":{"publication":"2026/08/29"},"accession":"GSE331378","cross_references":{"GSM":["GSM9744945","GSM9744934","GSM9744944","GSM9744933","GSM9744943","GSM9744932","GSM9744931","GSM9744942","GSM9744938","GSM9744949","GSM9744948","GSM9744937","GSM9744947","GSM9744936","GSM9744946","GSM9744935","GSM9744941","GSM9744952","GSM9744951","GSM9744940","GSM9744950","GSM9744939"],"GPL":["34281","34328"],"GSE":["331378"],"taxon":[" Mus musculus","Homo sapiens"]}}