GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE331nnn/GSE331455/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE331455GEOGSEfalsescRNA-seq of limb-mesenhymal organoid derived from mouse embryo during digit-like morphogenesisWe performed single-cell RNA sequencing of mouse embryonic limb mesenchymal cells and limb mesenchymal organoids during digit-like morphogenesis. Distal and proximal limb mesenchymal cells were isolated from E11.5 Slc:ICR mouse embryos, labeled using lipid-DNA CellPlex/Feature Barcode oligonucleotides, and profiled either immediately as Day0 samples or after culture as limb mesenchymal organoids under serum-only control or Fgf8b/Wnt3a-supplemented conditions. The dataset includes Day0 distal and proximal cells, Day1 and Day2 control organoids, and Day1 and Day2 Fgf8b/Wnt3a-treated organoids. These data were used to characterize distal and proximal cell states, treatment-dependent transcriptional changes, and lineage/state trajectories associated with self-organized digit-like morphogenesis.2026/05/24GSE331455GSM9746067GSM9746068GSM974606924247331455Mus musculus