{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE331nnn/GSE331509/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"species":["Mus musculus"],"gds_type":["Genome binding/occupancy profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE331509"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Limb cell-fate commissioning is guided by widespread coordinated genome detachment from the nuclear lamina [CUT&Tag]","description":"Diverse forms of heterochromatin block inappropriate transcription and safeguard differentiation and cell identity. Yet, how and when heterochromatin is reconfigured to facilitate changes in cell-fate remains a key open question. Here, we address this by mapping a prevalent heterochromatic feature - genome-lamina interactions - relative to transcription in single-cells during mouse embryogenesis. We find that lamina-genome interactions remain relatively uniform following gastrulation but are extensively reconfigured during organogenesis in diverse tissues. Focusing on limb development, we demonstrate that genome-lamina interactions are selectively released at key developmental genes and their surrounding regulatory domains in early multipotent progenitors. Strikingly, this “lamina-release” often precedes later gene expression, suggesting it primes regulatory domains for future potential activation. Lamina-release also coincides with the putative binding of crucial limb transcription factors and so is closely intertwined with the regulatory machinery that drives limb formation. Conversely, we show that CTCF-defined boundaries of topologically-associated domains (TADs) constrains the spread of lamina-release at a limb gene locus. This ensures independent heterochromatin dynamics between neighbouring domains. Together, this suggests a previously unrecognised process where genome-lamina interactions are selectively dismantled at regulatory domains to transition loci toward more permissive chromatin states, thereby potentiating cell-type specific activation. Our work thus reveals how systematic heterochromatin reorganization links to developmental multipotency, providing mechanistic insight into how progenitors traverse diverse cell-fates in vivo.","dates":{"publication":"2026/07/15"},"accession":"GSE331509","cross_references":{"GSM":["GSM9748327","GSM9748326","GSM9748329","GSM9748328","GSM9748323","GSM9748367","GSM9748366","GSM9748322","GSM9748369","GSM9748325","GSM9748324","GSM9748368","GSM9748363","GSM9748362","GSM9748321","GSM9748365","GSM9748320","GSM9748364","GSM9748361","GSM9748360","GSM9748338","GSM9748337","GSM9748339","GSM9748378","GSM9748334","GSM9748333","GSM9748377","GSM9748336","GSM9748335","GSM9748379","GSM9748330","GSM9748374","GSM9748373","GSM9748332","GSM9748376","GSM9748375","GSM9748331","GSM9748370","GSM9748372","GSM9748371","GSM9748349","GSM9748348","GSM9748345","GSM9748344","GSM9748347","GSM9748346","GSM9748341","GSM9748340","GSM9748343","GSM9748342","GSM9748381","GSM9748380","GSM9748359","GSM9748356","GSM9748355","GSM9748358","GSM9748357","GSM9748352","GSM9748351","GSM9748354","GSM9748353","GSM9748350"],"GPL":["24247"],"GSE":["331509"],"taxon":["Mus musculus"]}}