GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE332nnn/GSE332598/primaryOK200TranscriptomicsOrbilia oligospora ATCC 24927Expression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE332598GEOGSEfalseG protein influences spore and trap development, as well as energy metabolism and the cell cycle, in Arthrobotrys oligospora through the downstream cAMP and MAPK signaling pathwaysG proteins are conserved heterotrimeric proteins that serve as key components of G-protein signaling pathways and participate in various biological processes in fungi. In this study, we investigated the regulatory functions of the five G protein subunits in Arthrobotrys oligospora through gene knockout, phenotypic analysis, and transcriptomic profiling. Similar to most filamentous fungi, five G protein subunits were retrieved from A. oligospora, including three Gα subunits (GNA1, GNA2, and GNA3), a Gβ (GNB) and a Gγ (GNG) subunit. Our results demonstrated that gna2 and gng negatively regulate conidiation, whereas gna3, gnb, and gng are essential for[D1.1] conidiophore development and spore germination. Deletion of any G protein subunit resulted in severe defects in trap formation. Transcriptomic analysis further revealed that gna1 is involved in energy metabolism, while gng regulates cell cycle-related pathways and iron homeostasis in the strain. In addition, the relationship between G proteins and the MAPK and cAMP-PKA pathways was also investigated.2026/09/11GSE332598GSM9751319GSM9751308GSM9751309GSM9751322GSM9751300GSM9751311GSM9751323GSM9751312GSM9751301GSM9751302GSM9751313GSM9751303GSM9751314GSM9751315GSM9751304GSM9751305GSM9751316GSM9751306GSM9751317GSM9751318GSM9751307GSM9751320GSM9751321GSM975131036974332598Orbilia oligospora ATCC 24927