{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE332nnn/GSE332794/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"species":["Mus musculus"],"gds_type":["Genome binding/occupancy profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE332794"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Epigenomic profiling of H3K27ac in mouse brain regions under morphine and LPS exposure","description":"We profiled genome-wide H3K27ac chromatin modifications across four brain regions (hippocampus, prefrontal cortex, striatum, and hypothalamus) in male and female C57BL/6NTac mice using a perinatal morphine exposure model of Neonatal Opioid Withdrawal Syndrome (NOWS). Mice received morphine or saline perinatally, then were challenged with LPS or saline in adulthood, yielding four groups (SAL-SAL, SAL-LPS, MOR-SAL, MOR-LPS). NeuN+ neuronal nuclei (~10,000 per replicate) were isolated by FACS and profiled by MOWChIP-seq (Illumina NovaSeq X Plus, paired-end 150 nt). H3K27ac ChIP-seq identified differentially acetylated enhancers and promoters enriched in immune and metabolic pathways, with the hypothalamus exhibiting the most pronounced chromatin remodeling.","dates":{"publication":"2026/06/17"},"accession":"GSE332794","cross_references":{"GSM":["GSM9753519","GSM9753402","GSM9753523","GSM9753522","GSM9753401","GSM9753525","GSM9753404","GSM9753403","GSM9753524","GSM9753406","GSM9753527","GSM9753405","GSM9753526","GSM9753408","GSM9753529","GSM9753528","GSM9753407","GSM9753400","GSM9753521","GSM9753520","GSM9753509","GSM9753508","GSM9753512","GSM9753511","GSM9753514","GSM9753513","GSM9753516","GSM9753515","GSM9753518","GSM9753517","GSM9753510","GSM9753501","GSM9753500","GSM9753503","GSM9753502","GSM9753505","GSM9753504","GSM9753507","GSM9753506","GSM9753446","GSM9753567","GSM9753445","GSM9753566","GSM9753448","GSM9753569","GSM9753568","GSM9753447","GSM9753449","GSM9753440","GSM9753561","GSM9753560","GSM9753442","GSM9753563","GSM9753562","GSM9753441","GSM9753565","GSM9753444","GSM9753443","GSM9753564","GSM9753435","GSM9753556","GSM9753434","GSM9753555","GSM9753437","GSM9753558","GSM9753436","GSM9753557","GSM9753439","GSM9753559","GSM9753438","GSM9753550","GSM9753431","GSM9753552","GSM9753551","GSM9753430","GSM9753433","GSM9753554","GSM9753432","GSM9753553","GSM9753545","GSM9753424","GSM9753423","GSM9753544","GSM9753426","GSM9753547","GSM9753425","GSM9753546","GSM9753428","GSM9753549","GSM9753548","GSM9753427","GSM9753429","GSM9753420","GSM9753541","GSM9753540","GSM9753422","GSM9753543","GSM9753542","GSM9753421","GSM9753409","GSM9753413","GSM9753534","GSM9753412","GSM9753533","GSM9753536","GSM9753415","GSM9753414","GSM9753535","GSM9753417","GSM9753538","GSM9753416","GSM9753537","GSM9753419","GSM9753539","GSM9753418","GSM9753530","GSM9753411","GSM9753532","GSM9753531","GSM9753410","GSM9753480","GSM9753489","GSM9753482","GSM9753481","GSM9753484","GSM9753483","GSM9753486","GSM9753485","GSM9753488","GSM9753487","GSM9753479","GSM9753478","GSM9753471","GSM9753470","GSM9753473","GSM9753472","GSM9753475","GSM9753474","GSM9753477","GSM9753476","GSM9753468","GSM9753467","GSM9753469","GSM9753460","GSM9753462","GSM9753461","GSM9753464","GSM9753463","GSM9753466","GSM9753465","GSM9753457","GSM9753578","GSM9753456","GSM9753577","GSM9753459","GSM9753579","GSM9753458","GSM9753570","GSM9753451","GSM9753572","GSM9753571","GSM9753450","GSM9753453","GSM9753574","GSM9753452","GSM9753573","GSM9753455","GSM9753576","GSM9753454","GSM9753575","GSM9753390","GSM9753392","GSM9753391","GSM9753394","GSM9753393","GSM9753396","GSM9753395","GSM9753398","GSM9753397","GSM9753399","GSM9753381","GSM9753380","GSM9753383","GSM9753382","GSM9753385","GSM9753384","GSM9753387","GSM9753386","GSM9753389","GSM9753388","GSM9753491","GSM9753490","GSM9753379","GSM9753493","GSM9753492","GSM9753374","GSM9753495","GSM9753494","GSM9753376","GSM9753497","GSM9753496","GSM9753375","GSM9753499","GSM9753378","GSM9753377","GSM9753498"],"GPL":["34290"],"GSE":["332794"],"taxon":["Mus musculus"]}}