GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE332nnn/GSE332794/primaryOK200GenomicsMus musculusGenome binding/occupancy profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE332794GEOGSEfalseEpigenomic profiling of H3K27ac in mouse brain regions under morphine and LPS exposureWe profiled genome-wide H3K27ac chromatin modifications across four brain regions (hippocampus, prefrontal cortex, striatum, and hypothalamus) in male and female C57BL/6NTac mice using a perinatal morphine exposure model of Neonatal Opioid Withdrawal Syndrome (NOWS). Mice received morphine or saline perinatally, then were challenged with LPS or saline in adulthood, yielding four groups (SAL-SAL, SAL-LPS, MOR-SAL, MOR-LPS). NeuN+ neuronal nuclei (~10,000 per replicate) were isolated by FACS and profiled by MOWChIP-seq (Illumina NovaSeq X Plus, paired-end 150 nt). H3K27ac ChIP-seq identified differentially acetylated enhancers and promoters enriched in immune and metabolic pathways, with the hypothalamus exhibiting the most pronounced chromatin remodeling.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 musculus