{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE332nnn/GSE332798/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE332798"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Transcriptomic profiling of morphine and LPS exposure across mouse brain regions","description":"We profiled genome-wide gene expression across four brain regions (hippocampus, prefrontal cortex, striatum, and hypothalamus) in male and female C57BL/6NTac mice using a perinatal morphine exposure model of Neonatal Opioid Withdrawal Syndrome (NOWS). Mice received morphine or saline perinatally, then were challenged with LPS or saline in adulthood, yielding four groups (SAL-SAL, SAL-LPS, MOR-SAL, MOR-LPS). NeuN+ neuronal nuclei (~5,000 per sample) were isolated by FACS from PFC, hippocampus, hypothalamus, and striatum and profiled by RNA-seq (Illumina NovaSeq X Plus, paired-end 150 bp). RNA-seq identified differentially expressed genes enriched in immune and metabolic pathways, with the hypothalamus displaying the most pronounced transcriptional response to perinatal morphine exposure.","dates":{"publication":"2026/06/17"},"accession":"GSE332798","cross_references":{"GSM":["GSM9753644","GSM9753765","GSM9753643","GSM9753764","GSM9753767","GSM9753646","GSM9753645","GSM9753766","GSM9753648","GSM9753769","GSM9753647","GSM9753768","GSM9753649","GSM9753640","GSM9753761","GSM9753760","GSM9753642","GSM9753763","GSM9753641","GSM9753762","GSM9753629","GSM9753633","GSM9753754","GSM9753753","GSM9753632","GSM9753756","GSM9753635","GSM9753634","GSM9753755","GSM9753637","GSM9753758","GSM9753636","GSM9753757","GSM9753639","GSM9753759","GSM9753638","GSM9753750","GSM9753631","GSM9753752","GSM9753630","GSM9753751","GSM9753619","GSM9753739","GSM9753618","GSM9753622","GSM9753743","GSM9753621","GSM9753742","GSM9753624","GSM9753745","GSM9753623","GSM9753744","GSM9753747","GSM9753626","GSM9753625","GSM9753746","GSM9753628","GSM9753749","GSM9753627","GSM9753748","GSM9753620","GSM9753741","GSM9753740","GSM9753608","GSM9753729","GSM9753607","GSM9753728","GSM9753609","GSM9753611","GSM9753732","GSM9753610","GSM9753731","GSM9753613","GSM9753734","GSM9753733","GSM9753612","GSM9753736","GSM9753615","GSM9753614","GSM9753735","GSM9753617","GSM9753738","GSM9753616","GSM9753737","GSM9753730","GSM9753688","GSM9753687","GSM9753689","GSM9753680","GSM9753682","GSM9753681","GSM9753684","GSM9753683","GSM9753686","GSM9753685","GSM9753677","GSM9753676","GSM9753679","GSM9753678","GSM9753790","GSM9753671","GSM9753792","GSM9753670","GSM9753791","GSM9753673","GSM9753672","GSM9753675","GSM9753674","GSM9753787","GSM9753666","GSM9753665","GSM9753786","GSM9753668","GSM9753789","GSM9753667","GSM9753788","GSM9753669","GSM9753660","GSM9753781","GSM9753780","GSM9753662","GSM9753783","GSM9753782","GSM9753661","GSM9753664","GSM9753785","GSM9753663","GSM9753784","GSM9753776","GSM9753655","GSM9753654","GSM9753775","GSM9753657","GSM9753778","GSM9753656","GSM9753777","GSM9753659","GSM9753779","GSM9753658","GSM9753770","GSM9753651","GSM9753772","GSM9753650","GSM9753771","GSM9753653","GSM9753774","GSM9753773","GSM9753652","GSM9753590","GSM9753599","GSM9753592","GSM9753591","GSM9753594","GSM9753593","GSM9753596","GSM9753595","GSM9753598","GSM9753597","GSM9753589","GSM9753588","GSM9753585","GSM9753587","GSM9753586","GSM9753699","GSM9753698","GSM9753691","GSM9753690","GSM9753693","GSM9753692","GSM9753695","GSM9753694","GSM9753697","GSM9753696","GSM9753718","GSM9753717","GSM9753719","GSM9753600","GSM9753721","GSM9753720","GSM9753602","GSM9753723","GSM9753601","GSM9753722","GSM9753604","GSM9753725","GSM9753603","GSM9753724","GSM9753727","GSM9753606","GSM9753605","GSM9753726","GSM9753707","GSM9753706","GSM9753709","GSM9753708","GSM9753710","GSM9753712","GSM9753711","GSM9753714","GSM9753713","GSM9753716","GSM9753715","GSM9753701","GSM9753700","GSM9753703","GSM9753702","GSM9753705","GSM9753704"],"GPL":["34290"],"GSE":["332798"],"taxon":["Mus musculus"]}}