GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE332nnn/GSE332798/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE332798GEOGSEfalseTranscriptomic profiling of morphine and LPS exposure across mouse brain regionsWe profiled genome-wide gene expression across four brain regions (hippocampus, prefrontal cortex, striatum, and hypothalamus) in male and female C57BL/6NTac mice using a perinatal morphine exposure model of Neonatal Opioid Withdrawal Syndrome (NOWS). Mice received morphine or saline perinatally, then were challenged with LPS or saline in adulthood, yielding four groups (SAL-SAL, SAL-LPS, MOR-SAL, MOR-LPS). NeuN+ neuronal nuclei (~5,000 per sample) were isolated by FACS from PFC, hippocampus, hypothalamus, and striatum and profiled by RNA-seq (Illumina NovaSeq X Plus, paired-end 150 bp). RNA-seq identified differentially expressed genes enriched in immune and metabolic pathways, with the hypothalamus displaying the most pronounced transcriptional response to perinatal morphine exposure.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 musculus