{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE333nnn/GSE333160/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE333160"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Delayed processing of CPT-collected blood preserves transcriptomic and genomic features of propagated circulating tumor cells","description":"Circulating tumor cells (CTCs) provide a minimally invasive window into metastatic disease but are constrained by the need for rapid specimen processing after blood collection. We evaluated whether a workflow utilizing BD Vacutainer CPT-based blood collection could support delayed processing while preserving molecular features of propagated CTC-derived material. In a pilot cohort of four patients, paired CPT tubes were collected; one tube was processed within 2 hours of phlebotomy, whereas the second was stored at 4°C and processed 24 hours later. Both tubes were then harvested for CTCs, which were propagated ex vivo and analyzed by bulk RNA sequencing and whole-exome sequencing. Transcriptomic analyses showed that paired CPT-derived samples demonstrated phenotypes consistent with CTCs, including reduced immune-associated signatures and enrichment of epithelial-mesenchymal transition and KRAS signaling pathways. Direct comparison of paired early- and delayed-processed CPT samples demonstrated strong within-patient gene expression concordance. Whole-exome sequencing further revealed preservation of patient-specific oncogenic alterations and high overlap of detected variants across paired processing conditions. These findings altogether support the feasibility of a CPT-based delayed processing workflow for propagating and studying CTCs, thereby reducing a key logistical barrier to broader CTC research.","dates":{"publication":"2026/05/27"},"accession":"GSE333160","cross_references":{"GSM":["GSM9758120","GSM9758115","GSM9758114","GSM9758113","GSM9758112","GSM9758119","GSM9758118","GSM9758117","GSM9758116"],"GPL":["24676"],"GSE":["333160"],"taxon":["Homo sapiens"]}}