GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE333nnn/GSE333177/primary200OKTranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE333177GEOGSEfalseA 24-hour light-dark time-series bulk RNA-seq dataset of the mouse lacrimal glandThe lacrimal gland is a major exocrine organ responsible for tear secretion and is essential for maintaining ocular surface homeostasis. However, time-resolved transcriptomic resources for this tissue remain limited. Here, we generated a bulk RNA-seq time-series dataset from extraorbital lacrimal glands of male C57BL/6J mice maintained under a controlled 12:12 light-dark schedule. Tissue samples were collected at 3-h intervals across a 24-h cycle, yielding eight time points with three biological replicates per time point. Libraries were prepared from total RNA and sequenced as 150-bp paired-end reads. This dataset provides a reusable temporal transcriptomic resource for downstream studies of lacrimal gland biology, ocular surface physiology, and gene-expression dynamics under defined light-dark conditions.2026/06/22GSE333177GSM9758452GSM9758441GSM9758440GSM9758451GSM9758461GSM9758450GSM9758460GSM9758439GSM9758456GSM9758445GSM9758455GSM9758444GSM9758443GSM9758454GSM9758453GSM9758442GSM9758449GSM9758438GSM9758459GSM9758448GSM9758458GSM9758447GSM9758446GSM975845723479333177Mus musculus