{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE333nnn/GSE333477/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"species":[" Mus musculus","Homo sapiens"],"gds_type":["Genome binding/occupancy profiling by high throughput sequencing"," Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE333477"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Dynamicity of Topoisomerase 1 binding at super-enhancers regulates TLR-responsive gene expression program in macrophages","description":"Toll-like receptor (TLR) activation elicits an effective innate immune response by inducing the expression of primary and secondary response genes (PRGs and SRGs). Topoisomerase 1 (TOP1) has emerged as a critical regulator of innate immune responses; however, its mechanism of action remains largely unclear. Here, we demonstrate that ectopic expression of TOP1 is sufficient to program TLR-response in naïve macrophages and its catalytic activity is essential for inducible gene expression. TOP1 preferentially localizes to super-enhancers and TLR activation results in rapid redistribution of TOP1 from resting-state super-enhancers to stimulus-gained super-enhancers, leading to a transcriptional switch. Notably, TOP1 facilitates the recruitment of BRG1 to stimulus-gained super-enhancers. The cooperation between TOP1 and BRG1 enhances the chromatin accessibility at super-enhancers to preferentially regulate the expression of SRGs. These findings establish TOP1 as an epigenomic regulator that links chromatin remodeling at active super-enhancers with inducible gene expression in response to pathogenic stimuli.","dates":{"publication":"2026/05/28"},"accession":"GSE333477","cross_references":{"GSM":["GSM9765740","GSM9765741","GSM9765732","GSM9765699","GSM9765733","GSM9765734","GSM9765735","GSM9765736","GSM9765737","GSM9765738","GSM9765739","GSM9765691","GSM9765692","GSM9765693","GSM9765694","GSM9765695","GSM9765696","GSM9765730","GSM9765697","GSM9765698","GSM9765731","GSM9765690","GSM9765729","GSM9765721","GSM9765688","GSM9765722","GSM9765689","GSM9765723","GSM9765724","GSM9765725","GSM9765726","GSM9765727","GSM9765728","GSM9765680","GSM9765681","GSM9765682","GSM9765683","GSM9765684","GSM9765685","GSM9765686","GSM9765687","GSM9765720","GSM9765718","GSM9765719","GSM9765710","GSM9765677","GSM9765678","GSM9765711","GSM9765712","GSM9765679","GSM9765713","GSM9765714","GSM9765715","GSM9765716","GSM9765717","GSM9765670","GSM9765671","GSM9765672","GSM9765673","GSM9765674","GSM9765675","GSM9765676","GSM9765707","GSM9765708","GSM9765709","GSM9765666","GSM9765667","GSM9765700","GSM9765701","GSM9765668","GSM9765669","GSM9765702","GSM9765703","GSM9765704","GSM9765705","GSM9765706","GSM9765660","GSM9765661","GSM9765662","GSM9765663","GSM9765664","GSM9765665","GSM9765655","GSM9765656","GSM9765657","GSM9765658","GSM9765659","GSM9765650","GSM9765651","GSM9765652","GSM9765653","GSM9765654","GSM9765644","GSM9765645","GSM9765646","GSM9765647","GSM9765648","GSM9765649","GSM9765640","GSM9765641","GSM9765642","GSM9765643","GSM9765636","GSM9765637","GSM9765638","GSM9765639"],"GPL":["17021","24676","24247"],"GSE":["333477"],"taxon":[" Mus musculus","Homo sapiens"]}}