GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE333nnn/GSE333495/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE333495GEOGSEfalseSETDB1 regulates gene expression in mouse brown adipose tissue and primary brown adipocytesSETDB1 is a histone methyltransferase that catalyzes H3K9me3 and plays critical roles in transcriptional repression. To investigate its function in brown adipose tissue (BAT), we performed RNA-seq across three complementary models: (1) BAT tissue from BAT-specific Setdb1 knockout mice (Setdb1 AKO) vs control mice under thermoneutral conditions; (2) primary brown adipocytes from Setdb1 flox/flox; Rosa26Cre-ERT2 mice treated with 4-hydroxytamoxifen (4-OHT) vs vehicle (ETOH); and (3) primary brown adipocytes transfected with siRNA targeting Setdb1 (siSetdb1) vs non-targeting control siRNA (siNC).2026/06/06GSE333495GSM9766337GSM9766338GSM9766339GSM9766340GSM9766341GSM9766342GSM9766343GSM9766344GSM976634524247333495Mus musculus