{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE333nnn/GSE333596/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE333596"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Single-Cell RNA Sequencing Identifies Stem-Like Subpopulations and Their Gene Signature in Lung Adenocarcinoma Progression","description":"Lung adenocarcinoma (LUAD) exhibits substantial intra-tumor heterogeneity (ITH), contributing to disease progression and therapeutic challenges. This study utilized single-cell RNA sequencing (scRNA-seq) to profile cells from stage I and III LUAD tumors and matched normal tissues, aiming to delineate malignant cell states and identify tumor-propagating subpopulations. Unsupervised clustering revealed eight major cell lineages, with epithelial cells dominating in tumors and a shift toward immunosuppressive niches in advanced stages, including elevated T cell exhaustion and pro-angiogenic macrophages. Malignant cells formed 11 subclusters, with stage III tumors enriched for invasive and immunosuppressive states. Pseudotime analysis suggested evolutionary trajectories from alveolar-like to squamous and stress-responsive lineages. Using CytoTRACE to infer stemness without predefined markers, we identified sub-cancer stem cells (sub-CSCs) predominantly in stage III, characterized by high transcriptional diversity and a core signature involving cytoskeleton motility, immune modulation, metabolic reprogramming, and protein homeostasis. A four-gene panel demonstrated strong prognostic value in independent cohorts. These findings provide insights into stemness-driven progression in LUAD and nominate potential targets for addressing heterogeneity.","dates":{"publication":"2026/07/31"},"accession":"GSE333596","cross_references":{"GSM":["GSM9769648","GSM9769649","GSM9769650","GSM9769653","GSM9769654","GSM9769651","GSM9769652","GSM9769657","GSM9769658","GSM9769647","GSM9769655","GSM9769656"],"GPL":["24676"],"GSE":["333596"],"taxon":["Homo sapiens"]}}