{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE333nnn/GSE333645/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Pseudomonas putida KT2440"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE333645"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Global gene expression analysis of (R)-1,3-butanediol catabolism in Pseudomonas putida KT2440","description":"(R)-1,3-butanediol [(R)-1,3-BDO] is a chiral diol with potential applications in the chemical and pharmaceutical industries; however, its microbial degradation pathway remains poorly understood. In this study, we performed strand-specific, paired-end RNA-seq transcriptomic analysis of Pseudomonas putida KT2440 grown on (R)-1,3-BDO and (R)-3-hydroxybutyrate [(R)-3-HB] as sole carbon sources to elucidate the underlying degradation pathway. Gluconate was used as a reference carbon source. Differential gene expression analysis was performed to identify genes specifically induced during (R)-1,3-BDO and (R)-3-HB metabolism, providing insight into the enzymatic steps and regulatory mechanisms governing their catabolism in P. putida KT2440.","dates":{"publication":"2026/05/28"},"accession":"GSE333645","cross_references":{"GSM":["GSM9770345","GSM9770346","GSM9770341","GSM9770342","GSM9770343","GSM9770344"],"GPL":["37044"],"GSE":["333645"],"taxon":["Pseudomonas putida KT2440"]}}