GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE333nnn/GSE333645/primaryOK200TranscriptomicsPseudomonas putida KT2440Expression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE333645GEOGSEfalseGlobal gene expression analysis of (R)-1,3-butanediol catabolism in Pseudomonas putida KT2440(R)-1,3-butanediol [(R)-1,3-BDO] is a chiral diol with potential applications in the chemical and pharmaceutical industries; however, its microbial degradation pathway remains poorly understood. In this study, we performed strand-specific, paired-end RNA-seq transcriptomic analysis of Pseudomonas putida KT2440 grown on (R)-1,3-BDO and (R)-3-hydroxybutyrate [(R)-3-HB] as sole carbon sources to elucidate the underlying degradation pathway. Gluconate was used as a reference carbon source. Differential gene expression analysis was performed to identify genes specifically induced during (R)-1,3-BDO and (R)-3-HB metabolism, providing insight into the enzymatic steps and regulatory mechanisms governing their catabolism in P. putida KT2440.2026/05/28GSE333645GSM9770345GSM9770346GSM9770341GSM9770342GSM9770343GSM977034437044333645Pseudomonas putida KT2440